High-Resolution Melt PCR Analysis for Genotyping of Ureaplasma parvum Isolates Directly from Clinical Samples
نویسندگان
چکیده
منابع مشابه
High-resolution melt PCR analysis for genotyping of Ureaplasma parvum isolates directly from clinical samples.
Ureaplasma sp. infection in neonates and adults underlies a variety of disease pathologies. Of the two human Ureaplasma spp., Ureaplasma parvum is clinically the most common. We have developed a high-resolution melt (HRM) PCR assay for the differentiation of the four serovars of U. parvum in a single step. Currently U. parvum strains are separated into four serovars by sequencing the promoter a...
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Human infection with Chlamydophila (Chlamydia) psittaci can lead to psittacosis, a disease that occasionally results in severe pneumonia and other medical complications. C. psittaci is currently grouped into seven avian genotypes: A through F and E/B. Serological testing, outer membrane protein A (ompA) gene sequencing, and restriction fragment length polymorphism analysis are currently used fo...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2013
ISSN: 0095-1137
DOI: 10.1128/jcm.03036-13